elisa kits  (Elabscience Biotechnology)

Journal: Journal of Orthopaedic Surgery and Research

Article Title: GLP-2 alleviates postmenopausal osteoporosis by acting on osteoblasts through the PI3K/AKT/FOXO1 signalling pathway to downregulate FGF23 expression

doi: 10.1186/s13018-026-06661-2

Figure Lengend Snippet: Transcriptomic analysis of femoral tissues from sham, OVX, and OVX + GLP-2 groups. A Scatter plot of DEGs between the OVX and sham groups (1654 upregulated genes and 2038 downregulated genes). B Scatter plot of DEGs between the OVX + GLP-2 and OVX groups (318 upregulated genes and 396 downregulated genes). C Heatmap of 24 representative DEGs among the three groups. D GO enrichmentresults showing DEGs involved in cellular component, biological process, and molecular function categories. E KEGG signalling pathway diagram. F KEGG enrichment bar chart. G KEGG enrichment scatter plot. H Relative mRNA expression of FGF23, FGF6, and FGF18 in the tibiae of the OVX group

Article Snippet: FGF23 levels in cell culture supernatants were measured using a mouse FGF23 ELISA Kit (CSB-EL008629MO, Cusabio) following the manufacturer’s protocol.

Techniques: Expressing

Journal: Journal of Orthopaedic Surgery and Research

Article Title: GLP-2 alleviates postmenopausal osteoporosis by acting on osteoblasts through the PI3K/AKT/FOXO1 signalling pathway to downregulate FGF23 expression

doi: 10.1186/s13018-026-06661-2

Figure Lengend Snippet: Effects of GLP-2 and the PI3K inhibitor LY294002 on FGF23 expression in OVX mice. Forty-four female C57BL/6J mice were randomly divided into five groups: sham-operated (sham, n = 6), OVX treated with vehicle (OVX, n = 8), OVX treated with GLP-2 (OVX + GLP-2, n = 10), OVX treated with LY294002 (OVX + LY294002, n = 10), and OVX treated with GLP-2 plus LY294002 (OVX + GLP-2 + LY294002, n = 10). A IHC staining of FGF23 in the distal femur and corresponding MOD analysis. B Relative mRNA expression of FGF23 in femurs. C Serum FGF23 levels measured by ELISA. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: FGF23 levels in cell culture supernatants were measured using a mouse FGF23 ELISA Kit (CSB-EL008629MO, Cusabio) following the manufacturer’s protocol.

Techniques: Expressing, Immunohistochemistry, Enzyme-linked Immunosorbent Assay

Journal: Journal of Orthopaedic Surgery and Research

Article Title: GLP-2 alleviates postmenopausal osteoporosis by acting on osteoblasts through the PI3K/AKT/FOXO1 signalling pathway to downregulate FGF23 expression

doi: 10.1186/s13018-026-06661-2

Figure Lengend Snippet: GLP-2 downregulates FGF23 expression by activating GLP-2R/PI3K/AKT signalling in osteoblasts. A Western blot analysis of AKT and p-AKT levels in osteoblasts with or without shRNA-GLP-2R interference. B Relative mRNA expression of FGF23 in osteoblasts treated with or without shRNA-GLP-2R. C Western blot analysis of AKT and p-AKT levels in cells treated with or without LY294002. D Relative mRNA expression of FGF23 following LY294002 intervention. E Western blot analysis of AKT and p-AKT levels in cells treated with or without MK-2206. F Relative mRNA expression of FGF23 after MK-2206 treatment. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: FGF23 levels in cell culture supernatants were measured using a mouse FGF23 ELISA Kit (CSB-EL008629MO, Cusabio) following the manufacturer’s protocol.

Techniques: Expressing, Western Blot, shRNA

Journal: Journal of Orthopaedic Surgery and Research

Article Title: GLP-2 alleviates postmenopausal osteoporosis by acting on osteoblasts through the PI3K/AKT/FOXO1 signalling pathway to downregulate FGF23 expression

doi: 10.1186/s13018-026-06661-2

Figure Lengend Snippet: GLP-2 regulates FGF23 expression via the inactivation of FOXO1. A Western blot analysis of FOXO1 and p-FOXO1 levels in osteoblasts treated with or without GLP-2. B Immunofluorescence staining of FOXO1 in osteoblasts (green), counterstained with DAPI (blue). C Relative mRNA expression of FGF23 in osteoblasts treated with the FOXO1 inhibitor AS1842856. D Verification of FOXO1 overexpression by Western blot after PCDH-FOXO1 transfection. E Effect of FOXO1 overexpression on FGF23 mRNA levels in osteoblasts with or without GLP-2 treatment. F Effect of FOXO1 overexpression on the FGF23 concentration in the cell culture supernatant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: FGF23 levels in cell culture supernatants were measured using a mouse FGF23 ELISA Kit (CSB-EL008629MO, Cusabio) following the manufacturer’s protocol.

Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Over Expression, Transfection, Concentration Assay, Cell Culture

Journal: Journal of Orthopaedic Surgery and Research

Article Title: GLP-2 alleviates postmenopausal osteoporosis by acting on osteoblasts through the PI3K/AKT/FOXO1 signalling pathway to downregulate FGF23 expression

doi: 10.1186/s13018-026-06661-2

Figure Lengend Snippet: Mechanistic diagram illustrating how GLP-2 downregulates FGF23 to ameliorate postmenopausal osteoporosis via the GLP-2R/PI3K/AKT/FOXO1 signalling pathway

Article Snippet: FGF23 levels in cell culture supernatants were measured using a mouse FGF23 ELISA Kit (CSB-EL008629MO, Cusabio) following the manufacturer’s protocol.

Techniques:

Journal: JBMR Plus

Article Title: Genetic reduction or deletion of osteopontin in osteomalacic Hyp mice fails to ameliorate dentoalveolar defects

doi: 10.1093/jbmrpl/ziaf170

Figure Lengend Snippet: Total or partial ablation of the Spp1 gene does not alter blood biochemistry in Hyp mice. Blood biochemistry was analyzed at 60 dpn ( n = 4-5 mice/group). Calcium (Ca 2 + ) levels are unchanged across genotypes. Inorganic phosphate (P i ) levels are reduced equally across all Hyp groups regardless of Spp1 genotype. Elevated fibroblast growth factor 23 (FGF23) levels are unaffected by Spp1 status in Hyp mice. Circulating alkaline phosphatase (ALP) levels are similarly elevated across all Hyp mouse groups vs controls. PTH levels show a tendency to increase with either Spp1 deletion or in Hyp mice and Hyp;Spp1 −/+ mice show a significant increase over WT. Circulating inorganic pyrophosphate (PP i ) levels show no differences across groups. * p < .05; ** p < .01; *** p < .001; **** p < .0001.

Article Snippet: ELISA was performed to quantify levels of FGF23 using MicroVue mouse/rat FGF23 (C-term) ELISA kit (QuidelOrtho), and PTH levels were quantified by MicroVue mouse/Rat PTH 1-84 ELISA kit (QuidelOrtho).

Techniques:

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Fgf23 expression is down-regulated by pTxA2 in UMR-106 and MC3T3-E1 cells. Arithmetic means ± SEM of rel. Fgf23 gene expression in UMR-106 ( a , n = 5) and MC3T3-E1 ( b , n = 5) cells treated without or with pTxA2 for 24 h ( a ) or 6 h ( b ). * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Expressing, Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Fgf23 expression is decreased by TxA2 agonist I-BOP in UMR-106 and MC3T3-E1 cells. Arithmetic means ± SEM of rel. Fgf23 gene expression in UMR-106 ( a , n = 6) and in MC3T3-E1 ( b , n = 7) cells treated without or with TxA2 agonist I-BOP for 24 h ( a ) or 6 h ( b ). * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Expressing, Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Fgf23 expression is inhibited by TxA2 agonist U46619 in UMR-106 and MC3T3-E1 cells. Arithmetic means ± SEM ( n = 5) of rel. Fgf23 gene expression in UMR-106 ( a ) and MC3T3-E1 ( b ) cells treated without or with TxA2 agonist U46619 for 24 h ( a ) or 6 h ( b ). * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Expressing, Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: C-terminal FGF23 concentration in the supernatant of UMR-106 cells is lowered by TxA2 agonists. Arithmetic means ± SEM of C-terminal FGF23 concentration in the supernatant of UMR-106 cells treated without or with 100 ng/mL pTxA2 ( a , n = 7), 100 ng/mL I-BOP ( b , n = 7) or 5 µ m U46619 ( c , n = 8) for 24 h. * p < 0.05, ** p < 0.01. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Concentration Assay

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: TxA2 antagonist SQ29548 reverses the effect of I-BOP on Fgf23 mRNA transcripts. Arithmetic means ± SEM ( n = 10) of relative Fgf23 gene expression in UMR-106 cells treated without or with 100 ng/mL TxA2 agonist I-BOP in the presence or absence of 1 µ m TxA2 antagonist SQ29548 for 24 h. ** p < 0.01. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Exposure to activated platelets suppresses Fgf23 mRNA transcripts in UMR-106 cells. Arithmetic means ± SEM ( n = 8) of relative Fgf23 gene expression in UMR-106 cells exposed or not exposed to 100,000/µL freshly isolated human platelets in the presence or absence of 1 U/mL thrombin. * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Gene Expression, Isolation

Journal: bioRxiv

Article Title: Kidney cortex macrophages prevent the formation of mineral stones by reabsorbing phosphate from the urine

doi: 10.1101/2025.02.18.638798

Figure Lengend Snippet: ( A ) Scheme illustrating the generation of Slc34a1 ΔMØ mice and the experimental protocol of Panels B-E in which Slc34a1 ΔMØ mic were treated with either corn oil (Oil) or tamoxifen (TAM). ( B ) Efficiency of Slc34a1 abrogation in kidney cortex MØ, examined by RT-PCR. Each dot represents a pool of cells derived from 3 mice. ( C ) Urine output of the indicated electrolytes, adjusted by the excreted creatinine, before and after TAM treatment. ( D ) The concentrations of PTH and FGF23 in the blood plasma. ( E ) The relative expression of Cyp27b1 and Cyp24a1 transcripts in the kidney. ( F ) Scheme illustrating the protocol of low-phosphorus diet experiments for Panels G-I. ( G and H ) Urine Pi output (G) and Pi concentrations in blood plasma (H). ( I ) In open-field test, after the indicated treatments, Slc34a1 ΔMØ mice were placed in a square arena, and their total moving distance (left) and immobile time (right) were recorded during the observation period. ( J ) In rotarod test, after the indicated treatments, Slc34a1 ΔMØ mice were placed on a rod which then started to rotate in an accelerated speed. The on-rod time periods before their falling down were recorded. ns, not significant. * P <0.05, ** P <0.01, **** P <0.001 by two-tailed unpaired t test (B, D, E, I, J), two-tailed paired t test (C) and two-way ANOVA (G, H). Data are depicted as mean±SEM. Data are derived from at least 2 independent experiments.

Article Snippet: Plasma levels of PTH and FGF23 were measured by ELISA kits (Elabscience, #E-EL-M0709; Elabscience, #E-EL-M2415, respectively), following the manufacturer’s instructions.

Techniques: Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Expressing, Two Tailed Test